Topical Sulfur Mustard Induces Changes in Prostaglandins and InterLeukin-1 Alphain Isolated Perfused Porcine Skin

摘要

Su1fur mustard BIS(2-CHLOROETHYL) SULFIDE, HD is an alkylating agent that causessevere cutaneous injury. The isolated perfused porcine skin flap (IPPSF) is an in vitro model that has been utilized in cutaneous toxicity research. The objective of this study was to characterize the local IPPSF inflammatory response after topical exposure to 5.0 and 10.0 mg/ml of I (n = 5/treatment, n = 5/control). Biochemical markers of viability CUMULATIVE GLUCOSE UTILIZATION (CGU), vascular resistance (VR), morphological parameters, and venous flux of prostaglandin E2 (PGE2), prostaglandin F2% (PGF2%, and interleukin la (IL la)) were determined. HD caused a dose-related response in the formation of gross blisters, and epidermal-dermal separation. Decreases in CGU and an increase in VR were seen in all HD-treated IPPsFs. Increase of both PGE2 and PGF2a was observed only in 5.0 mg/ml HD treatment, which showed the greatest increase in VR, while the 10.0 mg/nil concentration of HD enhanced the release of IL-1a. These results suggest that HD is a potent dermal toxic agent that induces alterations in glucose metabolism and vascular resistance, which resulted in dose-specific patterns of PGE2, PGF2a and IL-la release.