LC-MS method for detecting prostratin in plant extracts and identification of a high-yielding population of Euphorbia fischeriana

摘要

Prostratin, a tigliane phorbol ester with promise for the treatment of HIV, was identified and quantified in Euphorbia fischeriana root extracts obtained from several different sites in China. The greatest yield was recovered from root samples collected from Yakeshi, Inner Mongolia (63.54 mu g/g dry weight, or 0.00635% by mass). Prostratin was not detected in extracts of Euphorbia sp., E. esula, E. lucorum or Stellera chamaejasme. The presence of prostratin was verified by H-1 and C-13 NMR. Major fragmentation products were identified from ESI MSn spectra after HPLC separation, providing a reproducible fingerprint for unambiguously recognizing the compound in plant extracts. (C) 2011 Phytochemical Society of Europe. Published by Elsevier B.V. All rights reserved.