Gibberellins in seedlings and flowering trees of Prunus avium L.

摘要

Extracts of acids from mature seeds, germinating seeds, first, second and third year seedlings, as well as mature, flowering trees of sweet cherry (Prunus avium cv. Stella) were analysed by gas chromatography-mass spectrometry. The presence of the known gibberellins (GAs) GA1, GA3, GA5, GA8, GA19, GA20, GA29, GA32, GA85, GA86 and GA87 was confirmed by comparison of their mass spectra and Kovats retention indices with those of standards or literature values. In addition, 16alpha,17-dihydrodihydroxyGA25 was identified and its stereochemistry confirmed by rational synthesis. The 12alpha,13-dihydroxy GAs, GA32, GA85, GA86 and GA87, were detected in mature seeds, germinating seeds and young seedlings, but not in flowering plants. The 13-hydroxy GAs, GA1 and GA3, were present in germinating seeds and, in addition to these, GA5, GA8, GA19, GA20 and GA29 were detected in seedlings and mature flowering plants. In germinating seeds and seedlings (while the plants were growing actively), concentrations ofthe 12alpha,13-dihydroxy GAs, measured by bioassay, declined and those of the 13-hydroxy GAs increased. The results are discussed with reference to the known and predicted effects of the GAs on the vegetative growth and flowering of P. avium plants.