GIBBERELLINS IN IMMATURE SEED OF PRUNUS CERASUS - STRUCTURE DETERMINATION AND SYNTHESIS OF GIBBERELLIN, GA(95) (1,2-DIDEHYDRO-GA(20))

摘要

Ten C-13-hydroxylated gibberellins (GA(3), GA(17), GA(19), GA(20), GA(32), GA(44), GA(86), GA(87), GA(95) and GA(95) isolactone) and two C-13-deoxy-GAs (GA(25) and GA(30)) were identified in immature seeds of sour cherry (Prunus cerasus L. cv. Montmorency) by GC-mass spectrometry. GA(95) is a new GA whose structure was determined to be 1,2-didehydroGA(20) by GC-mass spectral comparison with an authentic sample, synthesized from GA(3). In addition, six unknown GA-like compounds were detected by GC-mass spectrometry. Tt remains unclear whether GA(95) isolactone was an artefact. On the basis of the structures of the endogenous GAs and the absence of GA(5), the sequence of GA(20) --> GA(95) --> GA(3) --> GA(87) --> GA(32) could be conceived of as a possible biosynthetic pathway.