Stimulation of the production of hypericins by mannan in Hypericum perforatum shoot cultures.

摘要

Shoot organ cultures were established from callus derived from anthers of H. perforatum flowers. The effect of elicitors on hypericin and pseudohypericin production in shoot organ cultures was investigated. Mannan stimulated pseudohypericin production up to 4-fold (0.82 mg/g dry wt) and hypericin production up to 2-fold (0.04 mg/g dry wt). beta-1,3-Glucan and pectin slightly stimulated pseudohypericin production (～2-fold), but had no effect on hypericin production. Yeast extract showed no stimulatory effect on either hypericin or pseudohypericin production.