In cuticular waxes from leaves of Pisum sativum, 19 secondary alcohols, 10 primary/secondary alkanediols and three secondary/secondary alkanediols were identified by various chemical transformations with product assignment employing GC-MS. The homologous series of C(29)-C(33) secondary alcohols (1.1mug/cm(2)) was dominated by hentriacontanol isomers (94%). Only octacosanediols and trace amounts of hexacosanediols (<1%) were detected in the primary/secondary alkanediol faction (0.7mug/cm(2)). The secondary/secondary alkanediols (0.12mug/cm(2)) contained a single homologue with chain length C(31). All three compound classes showed characteristic isomer distributions with secondary functional groups predominantly located between C-14 and C-16. Based on the isomer compositions, the sequence of biosynthetic steps introducing the hydroxyl functions is discussed.
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