FRETView:a computer program to simplify the process of obtaining fluorescence resonance energy transfer parameters

摘要

The process of modeling the fluorescence resonance energy transfer (FRET) process for a donor-acceptor pair can be rather challenging,yet few computer programs exist that allow such modeling to be done with relative ease.In order to address this,we have developed a Java-based program,FRETView,which allows numerous FRET parameters to be obtained with just a few mouse clicks.Being a Java-based program,it runs equally well on all the major operating systems such as Windows,Mac OS X,Linux,Solaris.The program allows the user to effortlessly input pertinent information about the donor-acceptor pair,including the absorption and/or emission spectra,and outputs the calculated FRET parameters in table format,as well as graphical plots.