Effects of Organic Wastes, Glomus intraradices and Pseudomonas putida on the Growth of Tomato and on the Reproduction of the Root-knot Nematode Meloidogyne incognita

摘要

Effects of organic wastes (biosolids, horse manure, sawdust and neem leaf litter [NLL]), an arbuscular mycorrhizal (AM) fungus Glomus intraradices, and a plant growth-promoting rhizobacterium Pseudomonas putida, were studied on the growth of tomato and on the reproduction of Meloidogyne incognita. Pseudomonas putida and G. intraradices promoted tomato growth in nematode-infected and nematode-free plants but growth promotion was higher in the infected ones. When P putida and G. intraradices were applied together, the increase in tomato growth was greater than when either agent was applied alone. Of the organic wastes, NLL was better in improving tomato growth of nematode-infected plants followed by biosolids, horse manure and sawdust. Combined use of NLL with P putida plus G. intraradices was best in improving growth of the infected plants. Root colonization by P putida was increased more when inoculated with G. intraradices than when inoculated singly. Of the organic wastes, use of sawdust with P putida caused a greater increase in root colonization by fluorescent pseudomonads followed by NLL, horse manure and biosolids. Nematode parasitism had an adverse effect on root colonization by P putida. Inoculation of P. putida and organic wastes increased the root colonization caused by the AM fungus. P putida was better in reducing galling and nematode multiplication than G. intraradices, whereas use of the two together was better than that of either of them alone. Among organic wastes. NLL was better in reducing galling and nematode multiplication followed by biosolids, horse manure and sawdust. Combined use of NLL with P. putida plus G. intraradices was better in reducing galling and nematode multiplication than any other treatment.