Somatic embryogenesis and plantlet regeneration from cotyledon explants of Salvadora persica L.

摘要

High frequency somatic embryogenesis and subsequent plant regeneration were established from immature cotyledons of Salvadora persica L. Embryogenic calli were induced on Murashige and Skoog's medium supplemented with 2.5 mg/l 2,4-D and 0.5 mg/l Kn. Embryoids developed after 2-3 subculture on the same medium but with the addition of Proline at 20 mg/l. Somatic embryos matured on the above medium except proline, germination and optimal rooting was achieved on half strength MS medium devoid of any phytohormones but having 0.1% activated charcoal. The regenerated plantlets were successfully hardened and acclimatized in pots containing mixture of garden soil, manure and sand (1:1:1), with 65-70% survival rate in field.