...
首页> 外文期刊>Philosophical Transactions of the Royal Society of London, Series B. Biological Sciences >Antagonistic interactions in the zebrafish midline prior to the emergence of asymmetric gene expression are important for left-right patterning
【24h】

Antagonistic interactions in the zebrafish midline prior to the emergence of asymmetric gene expression are important for left-right patterning

机译:出现不对称基因表达之前斑马鱼中线的拮抗相互作用对左右模式很重要

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       

摘要

Left-right (L-R) asymmetry of the internal organs of vertebrates is presaged by domains of asymmetric gene expression in the lateral plate mesoderm (LPM) during somitogenesis. Ciliated L-R coordinators (LRCs) are critical for biasing the initiation of asymmetrically expressed genes, such as nodal and pitx2, to the left LPM. Other midline structures, including the notochord and floorplate, are then required to maintain these asymmetries. Here we report an unexpected role for the zebrafish EGF-CFC gene one-eyed pinhead (oep) in the midline to promote pitx2 expression in the LPM. Late zygotic oep (LZoep) mutants have strongly reduced or absent pitx2 expression in the LPM, but this expression can be rescued to strong levels by restoring oep in midline structures only. Furthermore, removing midline structures from LZoep embryos can rescue pitx2 expression in the LPM, suggesting the midline is a source of an LPM pitx2 repressor that is itself inhibited by oep. Reducing lefty1 activity in LZoep embryos mimics removal of the midline, implicating lefty1 in the midline-derived repression. Together, this suggests a model where Oep in the midline functions to overcome a midline-derived repressor, involving lefty1, to allow for the expression of left side-specific genes in the LPM.
机译:脊椎动物内部器官的左右(L-R)不对称是通过在体壁发生过程中侧板中胚层(LPM)中不对称基因表达域来预示的。纤毛L-R协调员(LRC)对于将不对称表达的基因(例如节点和pitx2)的起始偏向左LPM至关重要。然后需要其他中线结构(包括脊索和地板)来维持这些不对称性。在这里,我们报告中线中的斑马鱼EGF-CFC基因单眼针头(oep)的意外作用,以促进LPM中的pitx2表达。晚期合子oep(LZoep)突变体在LPM中的pitx2表达已大大降低或缺失,但仅通过在中线结构中恢复oep可以将这种表达恢复到强水平。此外,从LZoep胚胎中去除中线结构可以挽救LPM中pitx2的表达,这表明中线是自身受oep抑制的LPM pitx2阻遏物的来源。降低LZoep胚胎中的lefty1活性可模拟中线的去除,这牵涉到lefty1在中线衍生的抑制中。总之,这提示了一个模型,其中中线的Oep可以克服中线衍生的阻遏物(涉及lefty1),从而在LPM中表达左侧特异性基因。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号