Cascade-Like Modification of Immobilized Proteins Appiying a Magnetically Controlled Fluidic System

摘要

Besides their initial production in fer-mentative or cell-free Systems, pharma-ceutically relevant but also technical pro-teins often require posttranslational modifications. In the frame of the pro-ject "Selektive Kompartiment-Memb-ranen - in vitro Golgi-Apparat (Sele-KomM)", which is a part of the BMBF initiative "Biotechnologie 2020+", we are developing a compartmentalized reac-tion cascade allowing an automated, flexible modification of proteins via che-mical or enzymatic steps.