Bioguided Fractionation of Polygonum alpinum and Isolation and Structure Elucidation of Active Compounds

摘要

Bioguided fractionation of the methanol extract of aerial parts from Polygonum alpinum L (Polygonaceae) was investigated using the exudation phase of inflammation in aseptic arthritis model, which is produced by carra-geenan and radical-scavenging properties. Flavonoid-containing fractions showed anti-inflammatory and radical-scavenging activities. Therefore, isolation and structure elucidation of flavonoids were carried out. The eight flavonol glycosides, which were quercetin 3-O-arabi-nofuranoside (= avicularin) (1), quercetin 3-O-beta-glucuro-nopyranoside (2), quercetin 3-O-alpha-rhamnopyranosyl (1 -> 6)-beta-glucopyranoside (3), quercetin 3-O-beta-galacturo-nopyranoside (4), quercetin 3-O-beta-glucopyranoside (5), kaempferol 3-O-beta-galactopyranoside (6), quercetin 3-0-?galactopyranoside (=hyperoside) (7), and myricetin 3-o-beta-galactopyranoside (8), were isolated from the methanol herb extract of Polygonum alpinum. The structures were established by spectroscopic methods.