GC-MS analysis and antimicrobial activity of alkaloid extract from Genista vuralii

摘要

In the present study, the alkaloid composition of the aerial parts of Genista vuralii A. Duran & H. Dural (Fabaceae) was investigated by capillary GC-MS. Ten quinolizidine alkaloids were identified by capillary GC-MS, namely, N-methylcytisine,cytisine,tetrahydrorhombifoline, 17-oxosparteine, 5,6-dehydrolupanine, lupanine, 17-oxolupanine, anagyrine, baptifoline, and 13a-tigloyloxylupanine. Among them, anagyrine (93.04%) was the most abundant alkaloid. Furthermore, antibacterial and antifungal activities of the alkaloid extract of G. vuralii were tested against standard strains of bacteria (Escherichia coli, Pseudomonas aeru-ginosa, Bacillus subtilis, Staphylococcus aureus) as well as fungi (Candida albicans, Candida krusei). The alkaloid extract of G. vuralii presented good activity against S. aureus, B. subtilis, and C krusei, with minimum inhibitory concentrations (MIC) of 62.5 mug/mL.The remaining MIC values were found to range between 125 and 500 mug/mL To the best of our knowledge, the current work is the first to report the alkaloid profile and antimicrobial activity of G. vuralii L. growing in Turkey.