Immobilized bulk proteases in enzymatic resolution. Production of non-natural amino acids with high chiral purity

摘要

INTRODUCTION:The application of bulk enzymes for the production of chiral building blocks has increased the availability of these compounds, even on hundreds of tons scale. In combination with enzyme immobilization technology it has enabled the pharmaceutical industry to develop new routes to chiral intermediates and chiral APIs. In this article the preparation of non-natural isoleucine derivatives is taken as an example of how enzymatic resolution technology can be applied successfully.