Automatic determination of cell division rate using microscope images

摘要

Estimating the dynamics of cell culture development using fluorescent microscopy images is of great interest in modern cellular and molecular biology. In large-scale studies of cell populations involving a great number of images taken within a certain interval, manual analysis has proved to be time consuming and inaccurate; therefore, various computer-aided tools are required. This paper presents an efficient algorithm for recognizing budding cells and determining their division rate using microscope images. Image binarization using the Laplacian of the Gaussian; classifications of objects on cells, buds, and noise; and the determination of the cell division rate based on the data obtained are considered.