Isolation, identification and in vitro evaluation of endophytic actinomycetes against bacterial wilt pathogen in tomato

摘要

The efficacy of endophytic actinomycetes was tested against bacterial wilt pathogen in tomato under in vitro. They were isolated from tomato plants collected from five different locations in Kerala (India) and their antagonistic effects against bacterial wilt pathogen was evaluated under in vitro conditions. All the isolates belonged to genus Streptomyces. Maximum inhibition (29.25%) of the pathogen was observed with Vellanikkara isolate (EAVK) which was on par with Ozhalapathy isolate (EAOP) (22.59%) under in vitro conditions. The efficacy of culture filtrate of endophytic actinomycetes recorded maximum per cent inhibition (44.63%) of the pathogen with Cherumkuzhy isolate (EACK) which was on par with Ozhalapathy isolate (EAOP) (42.59%). It was concluded from the study that the Ozhalapathy isolate (EAOP) {Streptomyces sp.) was the most efficient among the endophytic actinomycetes isolates in the management of bacterial wilt pathogen under in vitro conditions.