DNA-based screening for an intracellular cadherin mutation conferring non-recessive Cry1Ac resistance in field populations of Helicoverpa armigera.

摘要

A number of cadherin mutants conferring resistance to Bt toxin Cry1Ac have been reported in three major lepidopteran pests, including Helicoverpa armigera. Unlike most of the cadherin mutants conferring recessive resistance, an allele (r₁₅) with a 55aa deletion in the intracellular domain of cadherin (HaCad) was previously identified to cause non-recessive resistance to Cry1Ac in H. armigera. In the present study, a DNA-based PCR method was developed to screen the r₁₅ allele from field populations of H. armigera collected from the main cotton planting areas of China in 2011 and 2012. Three heterozygous r₁₅ alleles were detected from 562 moths collected from northern China (with intensive Bt cotton planting), and r₁₅ allele frequency was estimated to be 0.0027. However, no r₁₅ allele was detected from 314 moths collected from Xinjiang (with limited Bt cotton use). Although all the r₁₅ alleles have the same deletion in the cDNA sequence, at least four different indels causing loss of exon 32 have been detected in the genomic DNA sequences flanking exon 32 of HaCad. Multiple origins of the r₁₅ alleles illustrate parallel genotypic adaption of H. armigera to the selection pressure of Bt cotton.