The drug transporter MgMfs1 can modulate sensitivity of field strains of the fungal wheat pathogen Mycosphaerella graminicola to the strobilurin fungicide trifloxystrobin

摘要

BACKGROUND:The major facilitator superfamily(MFS)drug transporter MgMfs1 of the wheat pathogen Mycosphaerella graminicola(Fuckel)J Schroeter is a potent multidrug transporter with high capacity to transport strobilurin fungicides in vitro.The data presented in this paper indicate that,in addition to the predominant cause of strobilurin resistance,cytochrome b G143A subsititution,MgMfs1 can play a role in sensitivity of field strains of this pathogen to trifloxystrobin.RESULTS:In a major part of field strains of M.graminicola(collected in the Netherlands in 2004)containing the cytochrome b G143A substitution,the basal level of expression of MgMfs1 was elevated as compared with sensitive strains lacking the G143A substitution.Induction of MgMfs1 expression in wild-type isolates upon treatment with trifloxystrobin at sublethal concentrations proceeded rapidly.Furthermore,in disease control experiments on wheat seedlings,disruption mutants of MgMfs1 displayed an increased sensitivity to trifloxystrobin.CONCLUSION:It is concluded that the drug transporter MgMfs1 is a determinant of strobilurin sensitivity of field strains of M.graminicola.