Genetic characterization of field-evolved resistance to phosphine in the rusty grain beetle, Cryptolestes ferrugineus (Laemophloeidae: Coleoptera)

摘要

Inheritance of resistance to phosphine fumigant was investigated in three field-collected strains of rusty grain beetle, Cryptolestes ferrugineus, Susceptible (S-strain), Weakly Resistant (Weak-R) and Strongly Resistant (Strong-R). The strains were purified for susceptibility, weak resistance and strong resistance to phosphine, respectively, to ensure homozygosity of resistance genotype. Crosses were established between S-strain x Weak-R, S-strain x Strong-R and Weak-R x Strong-R, and the dose mortality responses to phosphine of these strains and their F-1, F-2 and F-1-backcross progeny were obtained. The fumigations were undertaken at 25 degrees C and 55% RH for 72 h.