Acaricidal activities of paeonol and benzoic acid from Paeonia suffruticosa root bark and monoterpenoids against Tyrophagus putrescentiae (Acari:Acaridae)

摘要

The acaricidal activities of paeonol (2'-hydroxy-4'-methoxyacetophenone)and benzoic acid identified in the root bark of tree peony,Paeonia suffruticosa Andrews,against copra mite,Tyrophagus putrescentiae (Schrank),adults were examined using direct contact and vapour phase toxicity bioassays and compared with those of cinnamyl acetate,cinnamyl alcohol and 37 monoterpenoids as well as the acaricides benzyl benzoate,dibutyl phthalate and N,N-diethyl-m-toluamide (DEET).Based on LD_(50)values in fabric piece contact toxicity bioassays,the acaricidal activities of benzoic acid (4.80 mu g cm~(-2))and paeonol (5.29 mu g cm~(-2))were comparable to that of benzyl benzoate (4.46 mu g cm~(-2))but more pronounced than those of DEET (30.03 mu g cm~(-2))and dibutyl phthalate (25.23 mu g cm~(-2)).In vapour phase toxicity bioassays,paeonol and benzoic acid were much more effective in closed containers than in open ones,indicating that the effects of these compounds were largely due to action in the vapour phase.As judged by 24h LD_(50)values,(1S)-(-)-verbenone (7.42 mg per disc)was the most toxic fumigant,followed by (1S)-(-)-camphor,(S)-(+)-carvone,(R)-(-)-linalool and (+-)-camphor (10.45-18.18 mg).Potent fumigant toxicity was also observed with paeonol,(2S,5R)-(-)-menthone,(+-)-citronellal,benzoic acid,(1S,4R)-(-)-alpha-thujone and (R)-(+)-pulegone (25.10-34.63mg).Neither benzyl benzoate,DEET nor dibutyl phthalate caused fumigant toxicity.Paeonia root bark-derived materials,particularly paeonol and benzoic acid,as well as the monoterpenoids described,merit further study as potential acaricides or as leads for the control of T.putrescentiae.