Qualitative and quantitative differences in DNA extracted from different body parts of Apis spp. (Hymenoptera:Apidae) and its validation using microsatellite markers

摘要

To study the adaptability and the genetic diversity in Apis species, the molecular analyses play vital role for which good quality/ quantity of DNA is an important component. Here we describe a simple and efficient method for isolating quality genomicDNA from different body parts of Apis species through modifying the previous protocols. Alteration was made in the extraction buffer (100 mM Tris-HCl, pH 8.0, containing 2% Cetyltrimethylammonium bromide (CTAB), 1.5 M NaCl, 10 mM Ethylenediaminetetraacetic acid (EDTA) and 2% a-mercaptoethanol added after grinding) and the developed protocol was successfully tested on two different species, A. cerana and A.florea. DNA isolated from thorax yielded good quality DNA compared to other body parts that was further validated using microsatellite markers.