The production and comparative evaluation of native and recombinant antigens for the fast serodiagnosis of cystic echinococcosis with dot immunogold filtration assay

摘要

Clinical diagnosis and post-surgery assessment of cystic echinococcosis depend on laboratory serodiagnosis and ultrasound examinations. This study aims to produce the recombinant antigen (rAgB) and compare its diagnostic effect with natural antigens (crude fluid antigen, protoscolex antigen). After rAgB, crude fluid antigen, protoscolex antigen were produced, and the diagnostic accuracy was evaluated with dot immunogold filtration assay (DIGFA) by the sera from the following groups: surgically confirmed cystic echinococcosis patients (n=113), alveolar echinococcosis patients (n=46), other parasitic diseases (n=49), nonparasitic hepatic diseases (n=63) and healthy people (n=121). In diagnosing cystic echinococcosis, the sensitivity of recombinant AgB was 779% and the specificity was 983%. The crude fluid antigen B showed a sensitivity of 929% and specificity of 810%. The protoscolex antigen had sensitivity of 876% and specificity of 909%. The recombinant AgB indicates the advantage of no cross-reaction with other parasite diseases or nonparasite hepatic diseases. Recombinant antigen B can improve the specificity but decrease the sensitivity. The combination of native and recombinant antigens will improve the overall performance of serodiagnosis of cystic echinococcosis.