Clonal and genetic variation in three collembolan species revealed byisozymes and randomly amplified polymorphic DNA

摘要

Four strains or the collembolan Folsomia candida and one strain of F. fimetaria were analysed for genetic variation revealed by isozymes and randomly amplified polymorphic DNA (RAPD-PCR). Genetic variation for a strain of Hypogastrura assimilis was revealed by isozymes. Both analytical methods are applicable for separating strains of F. candida, and Ilo genetic variation within the strains of this parthenogenetic species was observed by either method. This observation strongly supported the applicability of F. candida as bioindicator like Daphnia magna. As expected for a sexually reproducing species F. fimetaria revealed genetic variation when applying either analytical method and so did H. assimilis when analysing isozymes. The four strains F. candida were clustering together in a taxon well separated from the two other collembolan species F. fimetaria and N. assimilis based on eight isozymes, representing nine loci.