Product Fermented by Lactobacilli Induces Changes in Intracellular Calcium Dynamics in Rat Brain Neurons

摘要

In this work we investigated the effect of concentrated metabolic products of lactobacilli (PP) on the dynamics of intracellular calcium concentration ([Ca~(2+)]_i) in rat brain neurons. [Ca~(2+)]_i was recorded using a fluorescent probe Fura-2 and a ratiometric Ca~(2+) imaging. It was found that PP increased [Ca~(2+)]_i, stimulating the intracellular signaling mechanisms. In these processes the activation of ryanodine receptors and protein kinase C are involved at least partially. Continuous application of PP stimulated a sustained release of Ca~(2+) from the endoplasmic reticulum and subsequent entry of Ca~(2+) into the cell. Given that PP is able to stimulate circulation and neurogenesis and is involved in calcium homeostasis in nerve cells in the brain, PP can be regarded as a product for the improvement of psychological parameters and cognitive functions of the brain.