ANTHELMINTIC ACTIVITY OF ADHATODA VASICA AGAINST GASTROINTESTINAL NEMATODES OF SHEEP

摘要

The anthelmintic activity of Adhatoda vasica was evaluated against the gastrointestinal nematodes of sheep through egg hatch and larval development tests in vitro and faecal egg counts reduction test in vivo. In vitro studies revealed that aqueous and ethanolic extracts at the concentration of 3.12, 6.3, 12.5, 25.0 and 50.0 mg/ml exhibited ovicidal and larvicidal effects (p < 0.05) against the eggs and larvae of gastrointestinal nematodes. The highest effective dose (ED50) value of A. vasica extract was recorded on the eggs of Chabertia ovina (18.20 mg/ml) with both aqueous and ethanolic extracts whereas the lower value was recorded on the eggs Ostertagia circumcincta (11.4 mg / ml) with ethanolic extract. Similarly, the higher ED50 value of A. vasica extracts was recorded against the larvae of Oesophagostomum columbianum (19.5 mg/ml) and the lower value against the larvae of H. contortus (12.88 mg/ml) with aqueous and ethanolic extracts respectively. In vivo studies revealed that experimental animal groups treated with the doses of 200 mg/kg of either aqueous or ethanolic extracts of A. vasica exhibited higher (p < 0.05) reduction rate on faecal egg counts (FEC) as compared to untreated groups (negative control). The highest reduction rate on FEC of treated animal groups recorded was 62.0 and 58.0 per cent with ethanolic and aqueous extracts respectively at the dose of 200 mg/kg on the 14(th) day post treatment whereas at the treatment doses of 50 and 100 mg/kg, the reduction rate ranged between 4.51 to 37.26 percent from 3(rd) onward to 14(th) day post treatment. The current study showed that A. vasica whole plant extracts possess anthelmintic activity, thus justifying their use in traditional and veterinary pratices.