Going from DNA Sequence to Protein Arrays

摘要

Protein arrays provide a powerful tool for large-scale studies on protein interactions,expression profiling and post-translational modifications.However,traditional cell-based expression methods for making protein arrays are generally a time-consuming process with a number of technical challenges.Cell-free protein synthesis offers an attractive system which not only allows rapid conversion of genetic information into functional proteins,but also provides flexible environments for producing proteins with correct folding or defined modifications.By parallel on-chip synthesis,it is now possible to make protein arrays directly from arrayed DNA templates.This article reviews the recent development in cell-free protein array technologies and their potential proteomics applications.