Human Embryonic Stem Cell Culture: The Next Step

摘要

Initial culturing techniques for human embryonic stem (hES) cells originated from methodology developed for murine ES cells,including the use of mouse embryonic fibroblast (MEF) feeder layers.While MEF feeder layers have proven to be a robust surface for long-term culture of hES cells,they present a number of limitations.Concerns about contamination of hES cells from animal-derived pathogens from MEF feeder layers makes this method of culturing unsuitable for clinical applications.