Picture in Picture

摘要

Atomic force microscopy(AFM)and optical microscopy,in particular fluorescence microscopy,make a powerful combination in the study of biological samples.AFM is not subject to Abbe's resolution limit,and can generate images with a much higher resolution than light microscopy.However,as contrast is generated in response to the structural properties of the sample,it can be challenging to detect specific structures in a heterogeneous sample,such as a cell.By combining the two techniques,higher resolution structural information can be generated using AFM.