SHG is an important contrast mechanism in optical examination for thick biological tissues. Fibrous proteins, such as myosin and collagen, exhibit bio-photonic crystal nature and are dominant SHG harmonophores in vertebrates. Local molecule arrangements strongly affect SHG polarization behavior. Here we demonstrate to distinguish myosin-based muscle fibers from intertwined collagenous perimysium through SHG polarization selection, without complicated staining or sample/image processing required.
展开▼
