Some years ago, when the new sequencing technologies entered the market, they were called "Next Generation Sequencers". The technical capability of the systems, particularly embodied by the Roche / 454 GS FLX and the Illumina / Solexa Genome Analyzer, generate huge amounts of sequence information (table l).The most important difference to traditional Sanger sequencing is the tremendous increase in data output. However, the read length of all Next-Gen machines which are commercially available, is shorter than the read length generated using the Sanger method. These two points: high data output and shorter read length, are the parameters which influence the bioinformatic analysis. To interpret and understand the biological relevance behind the data, Next Generation Bioinformatic services are required.
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