Indole Alkaloid Production in Callus Cultures of Uncaria rhvnchophvlla (MlQ.) MlQUEL

摘要

The effects of growth hormones and nutrients on growth and alkaloid production in callus cultures of Uncaria rhynchophylla (M_(IQ). M_(IQUEL) (Rubiaceae) were investigated. Gamborg B5(B5) medium supplemented with indoleacetic acid (10~(-4)M) and 6-benzylaminopurine (3*10~(-5)M) was optimized both for the growth concentration was examined, and 2% sucrose was found to be the most appropriate for the growth and alkaloid production. The effect of the composition of nitrogen sources was examined. Maximal growth and alkaloid production were obtained when 6mM ammonium chloride and 25mM potassium nitrate were added to the B5 medium. Hirsuteine, hirsutine, 3a-dihydrocadambine and ursolic acid were isolated from the callus. The 3a-dihydrocadambine concentration of callus was ca. 50-fold higher than that of the hooks and stems of U. rhynchophylla.