Triterpenes from the Spores of Ganoderma lucidum and Their Cytotoxicity against Meth-A and LLC Tumor Cells

摘要

Six new highly oxygenated lanostane-type triterpenes, called ganoderic acid γ (1), ganoderic acid δ (2), ganoderic acid ε (3), ganoderic aicd ζ (4), ganoderic acid η (5) and ganoderic acid θ (6), were isolated from the spores of Ganoderma lucidum, together with known ganolucidic acid D (7) and ganoderic acid C2 (8). Their structures of the new triterpenes were determined as (23S)-7β,15α,23-trihydroxy-3,11-dioxolanosta-8,24(E)-diene-26-oic acid (1), (23S)-7α,15α,23-trihydroxy-3,11-dioxolanosta-8,24(E)-diene-26-oic acid (2), (23S)-3β,7β,23-trihydroxy-11,15-dioxolanosta-8,24(E)-diene-26-oic acid (3), (23S)-3β,23-dihydroxy-7,11,15-trioxolanosta-8,24(E)-diene-26-oic acid (4), (23S)-3β,7β,12β,23-tetrahydroxy-11,15-dioxolanosta-8,24(E)-diene-26-oic acid (5) and (23S)-3β,12β,23-trihydroxy-7,11,15,-trioxolanosta-8,24(E)-diene-26-oic acid (6), respectively, by chemical and spectroscopic means, which included the determination of a chiral center in the side chain by a modification of Mosher's method. The cytotoxicity of the compounds isolated from the Ganoderma spores was carried out in vitro against Meth-A and LLC tumor cell lines.