Shivajiella indica gen. nov., sp. nov., a marine bacterium of the family 'Cyclobacteriaceae' with nitrate reducing activity

摘要

Novel orange pigmented, Cram-negative-staining, rod-shaped, non-motile, strictly aerobic strains designated NI0-S1~T and NIO-S2 were isolated from the water sample of a pond adjacent to the coast and an algal mat from a fish pond, respectively, at Kakinada, India. Both strains were positive for oxidase, catalase and beta-galactosidase activities. The predominant fatty acids in NIO-Sl~T were iso-C_(15:0) (39.6%), anteiso-C_(15:0) (9.9%), iso-C_(17:0) 30H (10.9%) and C_(16:1), omega 7c/C_(16:1) omega 6c (summed feature 3) (5.7%). The strains contained MK-7 as the major respiratory quinine, and diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and three unidentified lipids as the polar lipids. Phylogenetic analysis indicated that strain NIO-Sl~t was a member of the family "Cyclobacteriaceae" of the class "Sphingobacteriia" and it clustered with the genera Fontibacter, Cecembia and Aquiflexum with phylogenetic distances of 6.8, 9.0 and 12.2% (93.2, 91.0 and 87.8% similarity), respectively. DNA-DNA hybridization between strains NIO-Sl~t and NIO-S2 showed a relatedness of 93% and rep-PCR banding patterns were similar. Based on data from the current polyphasic study, it is proposed that the new isolates be placed in a new genus and species with the name Shivajiella indica gen. nov., sp. nov. The type strain of Shivajiella indica is NIO-Sl~T (- KCTC 19812~T = MTCC 11065~T).