Simultaneous Determination of Components in Preparation Naodesheng Injection by High Performance Liquid Chromatograph-Atmospheric Pressure Chemical Ionization Mass Spectrometry(HPLC-MS/APCI)

摘要

A high-performance liquid chromatograph with mass spectrum detection(HPLC-MS/APCI)method has been established for simultaneous determination of ten major bioactive components of Naodesheng injection including safflor yellow A,puerarin,daidzein,ginsenosides(Rg,,Rg_2,Rb_1,Rd,Re,Rh_1),and notoginsenoside R,.The separations were carried out with a Luna C_(18)column(5 mum,150X4.6 mm,Phenomenex,U.S.A.)with a stepwise gradient elution of the mobile phase consisting of water(0.1% of formic acid,v/v)-methanol(0 min,70:30;8 min,30:7020 min,10:90)at a flow-rate of 0.8 ml/min.The proposed method was applied to analyze five various Naodesheng injections and produced data with acceptable linearity,repeatability,precision and accuracy having lower limits of quantitation(LLOQs)of 0.02-0.2 mug.The calibration curves were linear in respective range for all compounds,all of them with coefficients of determination above 0.9900.The intraday precessions were less than 5.0%.The proposed method is accurate,sensitive and simple,a useful alternative for routine analysis in the quality control of Traditional Chinese Medicine.