Thin Layer Chromatographic Detection of Monocrotophos in Biological Samples

摘要

An organophosphorus insecticide monocrotophos is increasingly being used in agriculture to control insects on a variety of crops. Its ready access has resulted in misuse in many instances of homicidal and suicidal poisoning cases. This paper describes a chromogenic spray reagent for the detection/determination of monocrotophos. Monocrotophos on alkaline hydrolysis yields N-methyl acetoacetamide which in turn reacts with diazotized p-aminoacetophenone to give a red- violet colour. Other organophosphorus insecticides do not give a coloured spot. Moreover organochlorine and synthetic pyrethroid insecticides and constituents of viscera (amino acids, peptides, proteins etc), which are generally coextracted with the insecticides, do not interfere.