Structural analysis of protein-DNA and protein-RNA interactions by FTIR,UV-visible and CD spectroscopic methods

摘要

In this chapter the fundamental question of how does protein-DNA or protein-RNA interaction affect the structuresand dynamics of DNA, RNA and protein is addressed. Models for calf-thymus DNA and transfer RNA interactions with humanserum albumin (HSA), ribonuclease A (RNase A) and deoxyribonuclease I (DNase I) are presented here, using Fourier Trans-form Infrared (FTIR) spectroscopy in conjunction with UV-visible and CD spectroscopic methods. In the models considered,the binding sites, stability and structural aspects of protein-DNA and protein-RNA are discussed and the effects of proteininteraction on the secondary structures of DNA, RNA and protein were determined.