Production and characterization of digestion-resistant starch by the reaction of Neisseria polysaccharea amylosucrase.

摘要

Recombinant amylosucrase (200 U/mL) from Neisseria polysaccharea was used to produce digestion-resistant starch (RS) using 1-3% (w/v) corn starches and 0.1-0.5M sucrose incubated at 35 degrees C for 24 h. Characterization of the obtained enzyme-modified starches was investigated. Results show that the yields of the enzyme-modified starches were inversely proportional to the original amylose contents of corn starches. After enzymatic reaction, insoluble RS contents increased by 22.3 and 20.7% from 6.9% of waxy and 7.7% of normal corn starches, respectively, using 3.0% starch as acceptor and 0.3M sucrose as donor, while amylomaize VII showed the lowest increase (8.5%) in RS content. The crystalline polymorph of these enzyme-modified starches resulted in the B-type immediately after enzymatic reaction. The enzyme-modified starches displayed higher melting peak temperatures (85.6-100.6 degrees C) compared to their native starch counterparts (70.1-78.4 degrees C). After enzymatic reaction, pasting temperature increased in waxy (71.9 rightwards-arrow 77.6 degrees C) and normal corn starches (75.3 rightwards-arrow 80.6 degrees C), and the peak viscosity of waxy corn starches increased from 264 to 349 RVU, whereas that of normal corn starches decreased from 235 to 66 RVU. (c)WILEY-VCH Verlag GmbH & Co. KGaA. Weinheim