Near infrared diffuse reflection and laser-induced fluorescence spectroscopy for myocardial tissue characterisation

摘要

In order to evaluate the potential of cardiovascular tissue characterisation using near-infrared (NIR) spectroscopy. spectra in a previously unexplored wavelength region 0.8-2.3 mum were recorded from various pig heart tissue samples in vitro: normalmyocardium (with and without endo epicardium), aorta, fatty and fibrous heart tissue. The spectra were analysed with principal component analysis (PCA). revealing several spectroscopically characteristic features enabling tissue classification. Several of the identified spectral features could be attributed to specific tissue constituents by comparing the tissue signals with spectra obtained from water, elastin. collagen and cholesterol as well as with published data. The results obtained with the NIR spectroscopy technique in terms of its potential to classify different tissue types were compared with those from laser-induced fluorescence (LIF) using 337 nm excitation. LIF and NIR spectroscopy can in combination with PGA be used to discriminate between all previously mentioned tissue groups, apart from fatty versus fibrous tissue (LIF) and aorta versus fibrous tissue (NIR). respectively. The NIR analysis was improved by focusing the PCA to the wavelength segment 2.0-2.3 mum. resulting in successful spectral characterisation of all cardiovascular tissue groups.