A Study on Oxytetracycline-Human Serum Albumin Interaction Based on Flow-Injection Chemiluminescence Analysis Combined with Ultrafiltration Sampling

摘要

Binding properties of Oxytetracycline (OTC) and human serum albumin (HSA) have been studied in details.This investigation was based on flow injection Chemiluminescence (FI-CL) analysis and Ultrafiltration (UF) sampling technique.Chemiluminescence (CL) emission was observed during oxidation of OTC with KMnO_4 in acidic medium in the presence of formaldehyde as a sensitizer.Coupled with flow injection technique,OTC can be determined in the range of 8.0 x 10~(-7)-1.2 x 10~(-4) mol L~(-1).Oxytetracycline and HSA were mixed in different molar ratios and incubated at 37+-0.5 deg C.An Ultrafiltration probe was introduced to the sample containing unbound drug and the concentration of this free drug was determined by the CL method.The obtained data were analysed applying the Scatchard plot.The binding constant (K) and the number of binding sites (n) were 3.19 x 10~4 (mol L~(-1)) and 1.10,respectively.