A New Class of Arylamine Oxygenases:Evidence that p-Aminobenzoate /V-Oxygenase (AurF)is a Di-iron Enzyme and Further Mechanistic Studies

摘要

Arylamine oxygenases are enzymes that catalyze the unusual oxidation of an aryl amine group to an aryl nitro group.Although arylamine oxygenases seem to be widespread and used in diverse metabolic reactions,aminopyrrolnitrin oxy-genase (PrnD)and p-aminobenzoate oxygenase (AurF)are the only two known examples.Recently,PrnD was biochemically and mechanistically characterized.AurF,however,has had limited characterization,mainly due to the difficulty of obtaining functional enzymes in vitro.In addition,previous database searches (BLAST,PROSITE)failed to identify any known oxidoreductase cofactor binding sites or motifs in the AurF sequence;this indicated that AurF could be a novel class of arylamine oxygenases.