Fluorescent 5-Alkynyl-2'-Deoxyuridines:High Emission Efficiency of a Conjugated Perylene Nucleoside in a DNA Duplex

摘要

Four fluorescent 5-alkynyl~2f-deoxyuridines were studied in DNA oligonucleotides and their duplexes.The fluorescence response to hybridization differs dramatically for nucleosides containing a perylene fluorochrome either conjugated or not conjugated to the nucleobase.The conjugated nucleoside,5-(perylen-3-ylethyn-yl)-2'-deoxyuridine,shows enhanced long-wavelength emission in the DNA duplex,in contrast to the blue fluorescence of perylene on a flexible linker(in 5-[(perylen-3-yl)methoxyprop-1-ynyl]-2'-de-oxyuridine),which is quenched upon duplex formation.