Microelectrodes Integrated into a Microfluidic Chip for the Detection of CCRF-CEM Cells Based on the Electrochemical Oxidation of Hydrazine

摘要

We designed one type of microfluidic chip integrated with Au microelectrodes to detect target CCRF-CEM cells based on the electrochemical oxidation of hydrazine catalyzed by Pt nanoparticles (NPs). The microelectrode can improve the sensitivity of detection, with a low background signal and decreased nonspecific adsorption. Applying the cell aptamer as the sensing element, this method exhibits good selectivity. Owing to the strong catalytic ability of Pt NPs for the electrochemical oxidation of hydrazine, the limit of detection of this method is ten cells. By adjusting the detection area of Au microelectrodes, this chip can detect a wide range of target cells. Besides, this method successfully detected the target cells doped in blood samples, indicating its potential application in clinical diagnosis.