Characterization of human cyclooxygenase 2 gene promoter localization of a TGF-β response element

摘要

We characterized 2.3 kb of 5′-flanking region of the human cyclooxygenase 2 gene and analyzed its promoter activity. We localized three positive and two negative cis-acting regulatory regions and a TGF-β response region. Basal promoter activity was located between ?77 and + 99 bp. Maximal promoter activity was observed from ?1838 to +99 bp. A TGF-β response region was found between ?454 and ?288 bp. This region included an NF-I site. Electrophoretic mobility shift assays showed that TGF-β caused a marked change in the pattern and intensity of this NF-I like protein complex.