Qualitative and Quantitative Analysis of Hesperidin in Tablets by Thin Layer Chromatography with Densitometric UV Detection

摘要

Conditions for detection of hesperidin in pharmaceutical formulations,such as Hesperydynin tablets,have been established.Chromatographic separation was performed on 60 F_254 silica gel TLC plates with a chloroform-methanol-water (23+12+2,v/v/v) mobile phase.The spots were analysed by UV densitometry at LAMBDA approx = 286 nm.Under the established experimental conditions repeatable and accurate results were obtained.The method proposed is sensitive.It provides detection limit of 20 ng and satisfactory recovery in the range: 99.91%-100.74% for the declared content 100 mg.Linearity was maintained in the broad range from 4 to 44 ug mL~(-1).