Cloning and characterization of murine endothelial constitutive nitric oxide synthase

摘要

Complementary DNA clones encoding mouse endothelial constitutive nitric oxide synthase (ecNOS) were isolated by plaque hybridization from a murine fetal cardiac XZAP II cDNA expression library using a full-length human ecNOS cDNA as the hybridization probe, DNA sequence analysis indicates a 1202 amino acid protein showing significant sequence identity with human as well as bovine ecNOS.