A FlAsH-Tetracysteine Assay for Quantifying the Association and Orientation of Transmembrane α-Helices

Pace C.J.; Huang Q.; Wang F.; Palla K.S.; Fuller A.A.; Gao J.

首页> 外文期刊>Chembiochem: A European journal of chemical biology >A FlAsH-Tetracysteine Assay for Quantifying the Association and Orientation of Transmembrane α-Helices

【24h】

A FlAsH-Tetracysteine Assay for Quantifying the Association and Orientation of Transmembrane α-Helices

机译：FlAsH-Tetracysteine分析定量跨膜α-螺旋的缔合和方向

获取原文

获取原文并翻译 | 示例

掌桥外文数据库（机构版） >>

开具论文收录证明 >>

页面导航

摘要
著录项
相似文献
相关主题

摘要

Lighting up protein dimers in membranes: The association of membrane proteins plays a critical role in biology. We describe a FlAsH-tetracysteine (tC) assay that detects protein dimerization in lipid bilayers with a fluorescent readout. The stringent spatial requirement of FlAsH-tC complex formation readily differentiates helix dimers in parallel and antiparallel orientations.

机译：点亮膜中的蛋白质二聚体：膜蛋白质的结合在生物学中起着至关重要的作用。我们描述了一种FlAsH-四半胱氨酸（tC）分析，可检测具有荧光读数的脂质双层中的蛋白质二聚化。 FlAsH-tC复合物形成的严格空间要求很容易区分平行和反平行方向上的螺旋二聚体。

著录项

来源
《Chembiochem: A European journal of chemical biology》 |2011年第7期|共5页
作者
Pace C.J.; Huang Q.; Wang F.; Palla K.S.; Fuller A.A.; Gao J.;
展开▼
作者单位

展开▼
收录信息
原文格式 PDF
正文语种 eng
中图分类分子生物学;
关键词
Dimerization; FlAsH; Fluorescence; Membrane proteins; Transmembrane helices;

机译：二聚;FlAsH;荧光;膜蛋白;跨膜螺旋;
入库时间 2022-08-18 09:21:36

相似文献

外文文献
中文文献
专利

1. A FlAsH-Tetracysteine Assay for Quantifying the Association and Orientation of Transmembrane α-Helices [J] . Pace C.J., Huang Q., Wang F., Chembiochem: A European journal of chemical biology . 2011,第7期

机译：FlAsH-Tetracysteine分析定量跨膜α-螺旋的缔合和方向
2. Orientational Preferences of Neighboring Helices Can Drive ER Insertion of a Marginally Hydrophobic Transmembrane Helix [J] . ?jemalmK., HallingK.K., NilssonI., Molecular cell . 2012,第4期

机译：相邻螺旋的方向偏好可以驱动边缘疏水的跨膜螺旋的ER插入
3. Orientational Preferences of Neighboring Helices Can Drive ER Insertion of a Marginally Hydrophobic Transmembrane Helix [J] . ?jemalmK., HallingK.K., NilssonI., Molecular cell . 2012,第4期

机译：相邻螺旋的方向偏好可以驱动边缘疏水的跨膜螺旋的ER插入
4. Association-dissociation kinetics for dimerization of transmembrane helices as detected by single molecule fluorescence spectroscopy [C] . Yoshiaki Yano, Ryota Kitani, Katsumi Matsuzaki Japanese peptide symposium . 2011

机译：单分子荧光光谱检测的跨膜螺旋二聚化的联系 - 解离动力学
5. The Influence of Hydrophobic Mismatch on Structure and Dynamics of Transmembrane Helices and Lipid Bilayers [D] . Kim, Taehoon 2011

机译：疏水错配对跨膜螺旋和脂质双层结构和动力学的影响
6. Canonical Azimuthal Rotations and Flanking Residues Constrain the Orientation of Transmembrane Helices [O] . Orlando L. Sánchez-Muñoz, Erik Strandberg, E. Esteban-Martín, 2013

机译：典型的方位角旋转和侧翼残基限制了跨膜螺旋的方向
7. Self-association of transmembrane alpha-helices in model membranes - Importance of helix orientation and role of hydrophobic mismatch [O] . Sparr, Emma, Ash, W L, Rijkers, D T S, 2005

机译：跨膜α-螺旋在模型膜中的自缔合 - 螺旋取向的重要性和疏水性错配的作用
8. Development of Rapid In Vitro and In Vivo Assays to Detect and Quantify MYC Network Protein Associations [R] . Prochownik, E. V. 2002

机译：开发快速体外和体内检测以检测和定量mYC网络蛋白质关联

A FlAsH-Tetracysteine Assay for Quantifying the Association and Orientation of Transmembrane α-Helices

摘要

著录项

相似文献

相关主题

期刊订阅