Combined use of surfactant-induced coagulation of poly(allylamine hydrochloride) with peroxidase-mediated degradation for the rapid removal of estrogens and phenolic compounds from water

摘要

Sodium dodecyl sulfate (SDS)-induced coagulation of poly(allylamine hydrochloride) [PAH] was studied for the rapid removal of estrogens and phenolic compounds from water. When electrically equivalent amount (62 mg L~(-1)) of SDS was added into the aqueous solution of 20 mg L~(-1) PAH with vigorous mixing, PAH was quantitatively (>99%) recovered from water as condensed aggregates of PAH-SDS complexes. According to the fluorescence spectrum of a molecular probe, the complexes provided hydrophobic regions suitable for incorporating hydrophobic organic pollutants. However, rather polar estrone, (3-estradiol, estriol, and ethynylestradiol were insufficiently removed. Combined use of horseradish peroxidase (HRP) and hydrogen peroxide significantly increased the collection yields of these estrogens, because of their HRP-induced oxidation of and spontaneous binding to PAH. With the HRP activity of 100 U L~(-1) and the hydrogen peroxide concentration of 10 mg L~(-1), nearly complete (>98%) removal of four estrogens was achieved within 10 min at 30 °C in the pH region from 6 to 7.5. The method was also useful for the rapid removal of different phenolic compounds and selected pharmaceuticals. The applicability to wastewater treatment was successfully demonstrated by using secondary effluents.