Detection of biomolecules using optoelectronic biosensor based on localized surface plasmon resonance. Nanoimprint lithography approach

摘要

In this work, we use a nanoimprint lithography fabrication technology to create uniformly-oriented and homogenous noble metal nanoparticle arrays with well-controlled size, shape and spacing, which can be the basis platform for the development of Localized Surface Plasmon Resonance (LSPR) sensors for biomolecular detection. Using this approach, we demonstrate proof-of-principle of an optical biosensor to quantify biomolecular interactions in a real-time mode using a UV-visible spectrophotometer. The sensor shows concentration-dependent kinetics of surface adsorption or binding of biomolecules and a capability to monitor antigen-antibody specific reactions, which is demonstrated by the reaction between bovine serum albumin (BSA) and anti-BSA immunoglobulin.