Adsorptive stripping voltammetric determination of the anti-inflammatory drug tolmetin in bulk form, pharmaceutical formulation and human serum

摘要

The electro-reduction of tolmetin at the hanging mercury drop electrode was studied in different supporting electrolytes using cyclic voltammetry and square-wave stripping voltammetry techniques.Voltammograms of tolmetin exhibited a single well-defined 2-electron irreversible cathodic peak in media of pH<4,which may be attributed to reduction of the>C=O double bond of the analyte molecule.Adsorption of tolmetin onto the surface of the hanging mercury electrode was identified and each adsorbed tolmetin molecule was found to occupy an area of 0.23 nm~2.A square-wave adsorptive cathodic stripping voltammetric procedure was described for the direct determination of tolmetin in bulk form and pharmaceutical formulation(Rumatol~R capsules)with a limit of quantitation of 2×10~(-9) M and a mean percentage recovery of 98.35±1.21%to 99.57±1.23.Moreover,the described procedure was successfully applied for the direct assay of tolmetin in spiked human serum without pretreatment or extraction prior to the analysis while a quantitation limit of 5×10~(-9) M tolmetin was achieved.