Enzyme Biosensor Based on Hemoglobin Entrapped in Intestine-Like SnO_2 Nanostructure

摘要

A new morphology of SnO_2, intestine-like nano-cluster was employed for the fabrication of hydrogen peroxide biosensor. The prepared SnO_2 exhibited excellent biocompatibility and adsorption of hemoglobin, and the biosensor constructed by electro-codepositing of SnO_2 and hemoglobin on gold electrode exhibited sensitive response to the reduction of hydrogen peroxide, and the linear range was from 1 × 10~(-5) mol L~(-1) to 1.6 × 10~(-2) mol L~(-1) with coefficient constant of 0.99 (n = 23), and the detect limit was estimated to be 3.5 × 10~(-6) mol L~(-1) (S/N = 3). The selectivity, reproducibility and stability were also good.